Unable to encode due to lack of "Frame Rate info node"

[Selur]

SupTile is an Avisynth filter, so: No, Vapoursynth isn't using it.

Does the Vapoursynth Preview Windows show an error message?

The script itself looks like mine:

# Imports
import vapoursynth as vs
core = vs.get_core()
# Loading Plugins
core.std.LoadPlugin(path="I:/workspace/Hybrid/debug/64bit/vsfilters/SourceFilter/LSmashSource/vslsmashsource.dll")
# Loading F:\TestClips&Co\files\5000frames.mp4 using LWLibavSource
clip = core.lsmas.LWLibavSource(source="F:/TESTCL~1/files/5000FR~1.MP4", format="YUV420P10", cache=0)
# making sure input color matrix is set as 470bg
clip = core.resize.Point(clip, matrix_in_s="470bg",range_s="limited")
# making sure frame rate is set to 25/1
clip = core.std.AssumeFPS(clip, fpsnum=25, fpsden=1)
# Setting color range to TV (limited) range.
clip = core.std.SetFrameProp(clip=clip, prop="_ColorRange", intval=1)
# Loading image based subtitle F:\TestClips&Co\files\Subtitles\Aquaman.sup using Subtitle
subs = core.sub.ImageFile(clip=clip, file="F:/TestClips&Co/files/Subtitles/Aquaman.sup", blend=False)
alpha = core.std.PropToClip(subs)
subs = core.resize.Bicubic(subs, width=clip.width, height=clip.height, format=clip.format.id, matrix_s="470bg", range_s="limited")
gray_format = core.register_format(vs.GRAY, clip.format.sample_type, clip.format.bits_per_sample, 0, 0)
alpha = core.resize.Bicubic(alpha, width=clip.width, height=clip.height, format=gray_format.id, range_s="limited")
clip = core.std.MaskedMerge(clip, subs, alpha)
# adjusting output color from: RGB24 to YUV420P10 for x265Model (i420)
clip = core.resize.Bicubic(clip=clip, format=vs.YUV420P10, matrix_s="470bg", range_s="limited")
# Output
clip.set_output()

which works fine,..