Muxing interlaced video

[kingcrimsonster]

1.I am muxing the source file into the transport stream. As a muxer, I chose ffmpeg, since the ts-muxer introduces A-V resync. The output is a small inappropriate source file.
2. I am muxing the source file in to MKV.
In the tab "Muxing" - "Adjsut to stream frame rate" check or uncheck this box - no difference.
Source file options:

Format : MPEG Video
Frame rate : 25.000 FPS
Standard : PAL

Output File Options:

Frame rate mode : Variable
Frame rate : 50.000 FPS
Original frame rate : 25.000 FPS

If I mux the source file using mkvtoolnix - Output file options:

Frame rate mode : Constant
Frame rate : 25.000 FPS
Standard : PAL

Source file